User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 5: Line 5:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">AKAP250 (scbt) immunoblot AKAP250 Co-IP</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
Line 306: Line 306:
*Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
*Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
*Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|the ponceau of this membrane]]
*Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|the ponceau of this membrane]]
 
<!--
==Related==
==Related==
===Related entries===
===Related entries===

Revision as of 05:40, 29 July 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

AKAP250 (scbt) immunoblot AKAP250 Co-IP <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Washing the AKAP250 CO-IP Blot and adding secondary antibody
  • Adding AKAP250 antibody ZDE10030 animal 122 antibody (Eurogentech antibody service) to cAMP precipitation blot blocked in blocking buffer + 1 μL/mL Azide (20%stock)

Results

  • Immunoblot for AKAP250 on AKAP250 co-ip western blot

Discussion

  • Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
  • Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on the ponceau of this membrane


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12&oldid=437277"