User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/12: Difference between revisions

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==Notes==
==Notes==
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==Results==
==Results==
*{{todo| WHAT?}}
*Immunoblot for AKAP250 on AKAP250 co-ip western blot
[[Image:12072010_250co-ip_AKAP250_Blot.png|500px]]


==Discussion==
==Discussion==
*{{done}} Ow..
*Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
*Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|the ponceau of this membrane]]


==Related==
==Related==

Revision as of 05:47, 12 July 2010

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Summary

  • Washing the AKAP250 CO-IP Blot and adding secondary antibody
  • Adding AKAP250 antibody ZDE10030 animal 122 antibody (Eurogentech antibody service) to cAMP precipitation blot blocked in blocking buffer + 1 μL/mL Azide (20%stock)

Results

  • Immunoblot for AKAP250 on AKAP250 co-ip western blot

Discussion

  • Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
  • Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on the ponceau of this membrane

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