User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/03: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">ELISA, RII, IF</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
==Summary==
* ELISA (Caro & Mine)
* ELISA (Caro (12:08) & Mine (11:55))
** Mine was the same as the previous ([[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/01|01August2010]])
**NOTE: FORGOT TO ADD DMSO TO THE ONES NOT RECEIVING Ht31!
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Caro.100803'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''Control'''
| align="center" style="background:#f0f0f0;"|'''8Pcpt'''
| align="center" style="background:#f0f0f0;"|'''6Bnz'''
| align="center" style="background:#f0f0f0;"|'''Ht31 a.o.'''
| align="center" style="background:#f0f0f0;"|'''DMSO'''
| align="center" style="background:#f0f0f0;"|'''Cotransin'''
|-
|  || ||1||2||3||4||5||6
|-
| CTR||A||CTR ||CTR+8P ||CTR+6Bnz ||CSE+Ht31 ||DMSO+CTR ||Cotransin+CTR (2/400)
|-
| CTR||B||CTR ||CTR+8P ||CTR+6Bnz ||CSE+Ht31 ||DMSO+CTR ||Cotransin+CTR (6/400)
|-
| CSE||C||CSE ||CSE+8P ||CSE+6Bnz ||CSE+Ht31+6Bnz ||DMSO+CSE ||Cotransin+CSE (2/400)
|-
| CSE||D||CSE ||CSE+8P ||CSE+6Bnz ||CSE+Ht31+6Bnz ||DMSO+CSE ||Cotransin+CSE (6/400)
|}
* New cells were plated out, D9 P23
* RII Overlay showed NOTHING!! Not even marker signals...
** Most probable cause, blot was incubated ON up side down?
* IF Cells seeded in 100.000 cells per plate are swarming the coverslips, only use 75.000 cells from now on
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==Materials & Methods==
==Materials & Methods==

Revision as of 00:12, 4 August 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

ELISA, RII, IF <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • ELISA (Caro (12:08) & Mine (11:55))
    • Mine was the same as the previous (01August2010)
    • NOTE: FORGOT TO ADD DMSO TO THE ONES NOT RECEIVING Ht31!
Caro.100803 ' Control 8Pcpt 6Bnz Ht31 a.o. DMSO Cotransin
1 2 3 4 5 6
CTR A CTR CTR+8P CTR+6Bnz CSE+Ht31 DMSO+CTR Cotransin+CTR (2/400)
CTR B CTR CTR+8P CTR+6Bnz CSE+Ht31 DMSO+CTR Cotransin+CTR (6/400)
CSE C CSE CSE+8P CSE+6Bnz CSE+Ht31+6Bnz DMSO+CSE Cotransin+CSE (2/400)
CSE D CSE CSE+8P CSE+6Bnz CSE+Ht31+6Bnz DMSO+CSE Cotransin+CSE (6/400)
  • New cells were plated out, D9 P23
  • RII Overlay showed NOTHING!! Not even marker signals...
    • Most probable cause, blot was incubated ON up side down?
  • IF Cells seeded in 100.000 cells per plate are swarming the coverslips, only use 75.000 cells from now on


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