User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/11: Difference between revisions

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</html> [[Media:10082010_qPCR_AKAP12,_AKAP9.xls|qPCR Jurkat expression AKAP12, AKAP9, GAPDH]]
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Revision as of 04:50, 11 August 2010

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Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Western blot of yesterday was cut in 3 and incubated with primary antibody
    • AKAP450 (mouse, BD Bioscience, 611518)
    • RIIα(mouse, BD Bioscience, 612243)
    • AKAP95 (mouse, Transduction Laboratories, 610995)
  • GAPDH qPCR
    • As internal control also GAPDH need to be measured,
    • 20 µL reaction volume, as given by the machine
    • Human GAPDH primer (Applied biosystems)
    • Taqman Gene expression master mix

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<html> <a href="http://openwetware.org/wiki/Image:10082010_qPCR_AKAP12,_AKAP9.xls">

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</a> </html> qPCR Jurkat expression AKAP12, AKAP9, GAPDH