User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Summary== | ==Summary== | ||
* | * Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY | ||
* qPCR Patient material | |||
** NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions)) | |||
* Quantify loading and reload? | |||
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==Materials & Methods== | ==Materials & Methods== |
Latest revision as of 19:54, 26 September 2017
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
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