Wittrup: Dishwashing: Difference between revisions

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'''Glass bottles, glass flasks, Tunair flasks'''
'''Glass bottles, glass flasks'''
*wash in dishwasher
*wash in dishwasher (Cycle 1: Glassware)
*autoclave (sterilize 30 min, dry 30 min)
*autoclave (Cycle 5: 30 min sterilization, 30 min dry)
*place on shelves in tissue culture support room (76-286)
*place on shelves in tissue culture support room (76-286)
*NOTE: do not wash filters in Tunair caps


'''Centrifuge bottles'''
'''Centrifuge bottles, Tunair flasks'''
*wash in dishwasher
*wash in dishwasher (Cycle 6: Plastics)
*place on shelves in tissue culture support room (76-286)
*place on shelves in tissue culture support room (76-286)
*place some on table in main lab (76-291)
*NOTE: plastic bottles and flasks to be autoclaved as needed by user
              do not wash filters in Tunair flask caps
           


'''Spatulas, stir bars'''
'''Spatulas, stir bars'''
*wash in dishwasher
*wash in dishwasher (Cycle 1: Glassware)
*place on shelves in incubator / shaker room (76-284)
*place in containers on table in incubator / shaker room (76-284)


'''Pipet tips, microcentrifuge tubes, culture tubes'''
'''Pipet tips, microcentrifuge tubes, culture tubes'''
*autoclave (sterilize 30 min, dry 30 min)
*autoclave (Cycle 5: 30 min sterilization, 30 min dry)
*place on shelves in dishwasher / autoclave room
*place on shelves in dishwasher / autoclave room (76-292)
*place some on table in main lab (76-291)


'''Pasteur pipettes'''
'''Pasteur pipettes'''
*autoclave (sterilize 30 min, dry 30 min)
*autoclave (Cycle 5: 30 min sterilization, 30 min dry)
*place on shelves in tissue culture room
*place on shelves in tissue culture room (76-285)


'''Biohazard Waste'''
'''Biohazard Waste'''
*just before autoclaving, open bags to allow in steam
*autoclave (Cycle 1: 90 min sterilization)
*autoclave (sterilize 90 min)
*allowed to cool, then place each bag into a black plastic bag, secure, and set in hallway
*after autoclaving, tie up bags, place in black plastic bag (one per bag), place in hallway
*NOTE: just before autoclaving, open up waste bags to allow in steam during cycle


'''Biohazard Sharps'''
'''Biohazard Sharps'''
*empty bins are stored in the tissue culture support room (76-286)
*full bins are stored in the incubator / shaker room (76-284)
*the general accumulation area is room 76-268 (key on lab whiteboard)
*transport all full sharps bins to the accumulation room  
*transport all full sharps bins to the accumulation room  
*bring empty bins from the accumulation area to the tissue culture support room
*bring empty bins from the accumulation area to the tissue culture support room
*NOTE: empty bins are stored in the tissue culture support room (76-286)
              full bins are stored in the incubator / shaker room (76-284)
              the general accumulation area is room 76-268 (key on lab whiteboard)


'''Gel Buffers'''
'''Gel Buffers'''
* TAE, MES, MOPS, and transfer buffers are in room 76-287
*TAE, MES, MOPS, and transfer buffers are in room 76-287
*ensure all buffers are above refill line throughout the week
*ensure all buffers are above refill line throughout the week


'''Friday Check List'''
'''By the end of the day on Friday...'''
*glassware: washed, autoclaved, and shelved
*gel buffers: all tanks above refill line
*bio-sharps bins: full bins removed, replaced with empty bins
*unwashed glassware < 1 load
*unwashed glassware < 1 load
*biohazard waste < 2 bags
*biohazard waste < 2 bags

Revision as of 08:43, 22 March 2011

Glass bottles, glass flasks

  • wash in dishwasher (Cycle 1: Glassware)
  • autoclave (Cycle 5: 30 min sterilization, 30 min dry)
  • place on shelves in tissue culture support room (76-286)

Centrifuge bottles, Tunair flasks

  • wash in dishwasher (Cycle 6: Plastics)
  • place on shelves in tissue culture support room (76-286)
  • NOTE: plastic bottles and flasks to be autoclaved as needed by user
             do not wash filters in Tunair flask caps

Spatulas, stir bars

  • wash in dishwasher (Cycle 1: Glassware)
  • place in containers on table in incubator / shaker room (76-284)

Pipet tips, microcentrifuge tubes, culture tubes

  • autoclave (Cycle 5: 30 min sterilization, 30 min dry)
  • place on shelves in dishwasher / autoclave room (76-292)
  • place some on table in main lab (76-291)

Pasteur pipettes

  • autoclave (Cycle 5: 30 min sterilization, 30 min dry)
  • place on shelves in tissue culture room (76-285)

Biohazard Waste

  • autoclave (Cycle 1: 90 min sterilization)
  • allowed to cool, then place each bag into a black plastic bag, secure, and set in hallway
  • NOTE: just before autoclaving, open up waste bags to allow in steam during cycle

Biohazard Sharps

  • transport all full sharps bins to the accumulation room
  • bring empty bins from the accumulation area to the tissue culture support room
  • NOTE: empty bins are stored in the tissue culture support room (76-286)
             full bins are stored in the incubator / shaker room (76-284)
             the general accumulation area is room 76-268 (key on lab whiteboard)

Gel Buffers

  • TAE, MES, MOPS, and transfer buffers are in room 76-287
  • ensure all buffers are above refill line throughout the week

By the end of the day on Friday...

  • unwashed glassware < 1 load
  • biohazard waste < 2 bags
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