Wittrup: Dishwashing

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Glass bottles, glass flasks

  • wash in dishwasher on Glassware cycle
  • autoclave (30 min sterilization, 30 min dry)
  • place on shelves in tissue culture support room (76-286)

Centrifuge bottles, Tunair flasks, plastic beakers

  • wash in dishwasher on Plastics cycle
  • place centrifuge bottles and Tunair flasks on shelves in tissue culture support room (76-286)
  • place plastic beakers in incubator / shaker room (76-284)
  • NOTE:
    plastic bottles and flasks are to be autoclaved as needed by the user
    do not wash Tunair cap filters

Spatulas, stir bars

  • wash in dishwasher on Glassware cycle
  • place in containers on table in incubator / shaker room (76-284)

Pipet tips, microcentrifuge tubes, culture tubes

  • autoclave (30 min sterilization, 30 min dry)
  • place some on table in main lab (76-291)
  • place rest on shelves in dishwasher / autoclave room (76-292)
  • recycle empty tip holders in cardboard box
  • please empty cardboard box once full (recycling bin located in 76-270)

Pasteur pipettes

  • autoclave (30 min sterilization, 30 min dry)
  • place on shelves in tissue culture room (76-285)

Biohazard Waste

  • autoclave (90 min sterilization)
  • allowed to cool, then place each bag into a black plastic bag, secure, and set in hallway
  • NOTE:
    just before autoclaving, open up waste bags to allow in steam during cycle
    record tag numbers in biological waste log

Biohazard Sharps

  • transport all full sharps bins to the accumulation room
  • bring empty bins from the accumulation area to the incubator / shaker room
  • NOTE:
    empty bins are stored in the incubator / shaker room (76-284)
    full bins are stored in the incubator / shaker room (76-284)
    the general accumulation area is room 76-268 (key on lab whiteboard)

Gel Buffers

  • TAE, MES, MOPS, and transfer buffers are in gel / FPLC room (76-287)
  • ensure all buffers are the above refill line throughout the week

By the end of the day on Friday...

  • unwashed glassware < 1 load
  • biohazard waste < 2 bags
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