840:153g:Projects/project25/2012/10/18

Cloning of mreB from Caulobacter crescentus into E. coli

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Experiment 5 Preparation This week we worked on preparing for plasmid transformation using our primary plasmid (PSB1A3) and our alternative plasmid (PSB2K3). We made LBA and LBA with ampicillin plates for our PSB1A3 plasmid. The PSB2K3 plates were previously made (with kanamycin) and supplied by our professor. The PSB2K3 plasmid has already been transformed; we will only need to streak it onto the plates when we come to that point with our PSB1A3 plasmid. Ampicillin selects for PSB1A3 and kanamycin selects for PSB2K3. We also performed extraction of PSB1A3 from Parts Registry 2011 Distribution Kit. This will allow us to start transformation next week.