840:153g:Projects/project9/2010/09/30

• We attempted to extract the red florescent biobrick dna containing plasmid by following the bio brick procedure.
• The biobrick was not successfully isolated as no bacterial colonies grew.
• We prepped our primers for PCR and ran a gradient of temperatures, 44,47.8,51.4,56.2,59.9,64 in the PCR machine to discover our ideal annealing temperature.
• Primers were left to run the PCR cycle and then put in the -20 freezer.