AU Biomaterials Design Lab:Lab Members/CHEM571:Notebook/Chem 471- Fall 2018/2018/09/05

Project name

Main project page Previous entry      Next entry

08/30/2018-Experimenting with Aliphatic Amino Acids and NaOH Only Methionine showed a strong shift, though it turned black- no gold nanoparticles but at least it was reactive. This could be because of the sulfur in the R-group. From this we planned a pH assay with both Methionine and Cyestine (which also has a sulfur). For an acidic component: HCl Stock HCl = 36.5% solution --> halved --> 18.25 % --> 9.125% solution --> 2mL stock solution/ 6mL DI water 36.46 g/mol --> MW of HCl pH Assay: Acidic- 5mM HCl Control- no pH modifiers Basic- 1.25 mM NaOH 2.5 mM NaOH 5 mM NaOH 7.5 mM NaOH Two assays: one using Meth one using Cys 2mL Gold solution 2mL Base/Acid 2mL Amino Acid 4 hours in an 80℃ oven- samples were looked at the following Tuesday (9/11/18) Biggest Changes: Meth 5mM NaOH- deep red Cys 5mM NaOH- Grey/blue precipitated out