During the last lab period of this series, each group will make a short oral presentation to the rest of the class on a current cell imaging method and its application to studies of the cytoskeleton. Choices of topics may include:
- confocal microscopy
- electron microscopy (EM)
- electron tomography
- atomic force microscopy (AFM)
- fluorescent speckle microscopy
- photoactivated localization microscopy
- optical trapping/optical tweezers
- nuclear magnetic resonance (NMR)
- X-ray crystallography
- FRET (fluorescence resonance energy transfer)
- FRAP (Fluorescence recovery after photobleaching)
Your instructor will provide a sign-up sheet once you and your partners have decided which tool you would like to investigate so that each group will present something different. One person in each group should present general background information about the method (history and how it works), and the other group members should present the relevant parts of the chosen research article (background, objective, methods, data figure(s), conclusions). You do not need to present all the data in the paper, just the data obtained using your imaging method. Each presentation should: use Power Point, last approximately 10 minutes, include visual images with proper citations, and have a slide with the complete reference information for all of the sources you use for its preparation. All presentations should be e-mailed to your instructor immediately before the final lab session. The group presentation will be worth 25 points and will have both a group and an individual graded component. Evidence of collaborative work and advance practice as a group will be components of the group grade.
| Parameters || Explanation || Possible Points & Comments || Points Earned
| Introduction to Imaging Technique
|| Pertinent, reliable background information on the imaging technique chosen & presented. Demonstrated understanding of conceptual information. Imparted essentials to listeners clearly & succinctly. Avoided overly detailed tangential information or inappropriate information. Credit given for all sources.
Posture, voice, eye contact, timing, & organization facilitated listener understanding.
| Research Article Presentation
|| Choice of research report to present was appropriate. Presented a brief overview of topic and experimental goals and methods in appropriate detail.
Centered presentation around experimental findings using the imaging method; used appropriately labeled high quality data images and explained the authors’ findings accurately and clearly. Drew conclusions from the images presented clearly & succinctly; explained relationship between structure & cellular function, if appropriate. Posture, voice, eye contact, timing, & organization facilitated listener understanding.
Credit given for all sources.
| Group Demonstrated Knowledge of Content
|| Demonstrated understanding of information. Answered questions accurately & with poise & confidence.
| Group Quality of Presentation
|| Group demonstrated overall understanding of assignment; parts complemented whole; presentation was smooth, not repetitive & appeared practiced & of length assigned. Used high quality visual aids.
|| Combines Individual (15 points) & Group Points (10points)