BISC220/S11: Media Recipes

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Apoptosis Series Media Recipes

Solution Composition
PBS (Phosphate Buffered Saline) 1X 2.68 mM KCl
1.46 mM KH2PO4
136.9 NaCl
8.1 mM Na2HPO4
PBST (PBS with Tween 20) 1X PBS with 0.25% Tween 20
Trypsin Stock = 0.25%
Cell Culture Media 1 Packet DMEM Powder
0.15% NaHCO3
10% Newborn Calf Serum
Trypan Blue 0.1% Trypan Blue in water
Etoposide 100 mM in DMSO (stock)
500 μM (effective)
DNA extraction buffer 50 mM Tris pH 8.0
20 mM Na2EDTA
10 mM NaCl
1% SDS
20 ug/ml RNase A
PMSF/glucose buffer (protein buffer 1) 50 mM glucose
25 mM Tris pH 8.0
10 mM Na2EDTA
and 1 mM PMSF
urea/SDS buffer (protein buffer 2) 50 mM Tris pH 6.8
6M urea
6% B-ME
3% SDS
0.003% bromophenol blue
anti-PARP-1 antibody (mouse) diluted 1:1000
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