BISC220/S14:Series 1

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Wellesley College     BISC 220     Cellular Physiology

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Schedule of Experiments

Lab # Dates Activity Outside lab time
1 1/27 - 1/31 Introduction to BISC220 Lab
2 2/3 - 2/7 1) Induction of β-galactosidase
2) Molecular Modeling and Sequence Analysis
Figure creation and brief analysis of the β-galactosidase molecule
3 2/10 - 2/14 1) Affinity Chromatography
2) Polyacrylamide Gel Electrophoresis
Figure Design and Legend Writing
4 2/18- 2/24 Determination of the Specific Activity of β-galactosidase Calculations based on the results of today's lab
5 2/25 - 3/3 Enzyme Kinetics 1) Calculations based on the results of today's lab

2) Prepare for writing workshop

6 3/4 - 3/10 Writing Workshop Write Final Paper for Enzyme Kinetics