BISC 219/F10: Assignment Series2 Complementation

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Lab 5 Complementation Assignment

Complementation analysis is an important and widely used genetic tool. We will use it to confirm and expand our knowledge about the gene mutation that is responsible for the phenotype abberation you are studying. You should find and read some general background information before you start writing this assignment. A good place to start is the Wikipedia article found at: [1]. An excellent scholarly article by Hawley and Gilliland can be found in Genetics, Vol. 174, 5-15, September 2006, at doi:10.1534/genetics.106.064550. Your homework assignment to show that you understand complementation and how you will use it in our Series 2 forward genetics project will be due at the beginning of lab next time.

Please write a 1-2 page summary describing complementation analysis as a genetic tool both generally and specifically related to your project. You will probably need to use and cite outside sources (NOT Wikipedia!). Please use the journal Genetics as a model for in text and reference page cition style. You must paraphrase information from sources (no direct quoting in any work submitted for BISC219) and you must not cite Wikipedia or the lab wiki since they are secondary sources and not peer-reviewed. Use Wikipedia initially to learn more about your subject and to find appropriate primary sources to cite. Since you are using sources and citing them in-text, an appropriately formatted Literature Cited page is required as part of this assignment. Include diagrams of the crosses you will set up for complementation analysis from beginning to end including expected results when your mutation is allelic and non-allelic to a reference mutation. Use templates provided.

Grading Rubric

Assignment Complementation Analysis 5 points

At or Above Standard Below Standard Possible
Points
Points

Earned

Narrative summary of complementation in general Constructed a complete, clear, concise, and accurate summary of what complementation is and how it is used as a genetic tool; Use appropriate sources and cited sources correctly in proper Genetics style Information is inaccurate or only partially inaccurate; Contains too much tangential information or is too simplified; Missing essential information; References not cited, not cited appropriately or in proper format (Genetics, Name/Year style). Omited Literature Cited page or formatted it incorrectly. 1 __/1
Narrative summary of complementation specifically related to Series 2 Forward Genetics Project
Constructed a clear, concise, accurate, and logically developed description of the role of complementation testing in our experimental approach to locating and characterizing a gene mutation responsible for a phenotypic alteration from wild type Essential information missing, inaccurate, partially accurate; Contains too much tangential information or is too simplified, Information isn't logically developed 2 __/2
Cross diagrams
Used templates provided to draw out accurate and clear predictions of crosses set up for complementation analysis and expected results when unknown mutation was allelic and non-allelic to reference mutations. Crosses missing, inaccurate, or only partially accurate. 2 __/2
Total 5 __/5



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