BME100 f2018:Group4 T0800 L6

BME 100 Fall 2018	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Alexander Sedlack Role(s) Name: Gabe Zdrale Role(s) Name: Dalia Khaled Role(s) Name: Collin Conners Role(s) Name: Jason Shenon Role(s) Our Brand Name LAB 6 WRITE-UP Bayesian Statistics Overview of the Original Diagnosis System What Bayes Statistics Imply about This Diagnostic Approach The relationship between the results from the PCR reaction and the test conclusion were to 1.0 (100%), and therefore were seen to be highly accurate. Although this can be a very reliable source used to detect disease, you cannot heavily rely on only using this method in order to conclude whether the disease is present or not. The reliability of this method that is used to whether the disease is present in the organism or not, ultimately is not very accurate due to the fact that the average of the values approximated to about 0.6 (60%). Therefore, this method is not very reliable and you cannot correctly or confidently determine whether or not the disease was present in the organism. There are many possible sources of error that could have occurred during this lab. One possible source of error includes that there could have been an error in the ability of the phone camera to take a high quality picture. If the pictures from the phone camera were not accurate enough, then the ImageJ would not be able to detect the fluorescence in the droplet as well. Most of the phone cameras do not have the best ability to adjust the individual settings in order to create the best possible picture. Another possible source of error could be our inexperience with the equipment especially the micropipette. Since many of us do not have the experience handling the micropipette before this lab, many of us could have made mistakes inputting and extracting the various PCR samples into the separate test tubes. Our inexperience with the micropipette may have caused us problems, not having the correct amount of samples, effecting our results later in the lab. Another possible source of error could have been the excess light and the lack of darkness in the box. Since the lid of the box containing the fluorescence had to be slightly opened in order to take a picture, some light could have peeped through into the box, effecting the image and the results of the samples. The light could have decreased the green fluorescence in the samples, making our results and images less accurate. Intro to Computer-Aided Design 3D Modeling During the Computer-Aided Design (CAD) lab Solidworks was used to replicate the construction of our new idea. The idea chosen was similar to the preexisting design. Though a bit difficult to use, Solidworks allowed for small details and was much simpler than trying to make a real model. Solidworks is predominantly a professional software and thus the experience becomes more enjoyable the more skilled one gets from practice. Although Solidworks is still preferred because of its control and detail. Our Design The design pictured above is very similar to the previous design used in the PCR experiment, however, it features a 5 by 5 tray instead of a 4 by 4 tray allowing for greater efficiency and less need to clean the tray or provide a new one. This design is similar enough to the tried and true design used in the experiment that it should not fail and also perform with greater efficiency because of the extra space provided on the tray. Feature 1: Consumables PCR consumables are essentially plastics that are required to carry out the PCR reaction through the thermal cycler. These items are designed to optimize PCR performance and storage. The most important consumables include plates, tubes, and caps with heat sealers to prevent evaporation from the PCR plates. Our redesigned PCR machine maintains the fit for standard tubes that can be found at Thermofisher. This company includes various types of tubes for different reactions such as standard dimension color tubes, tubes with barcodes, PCR inhibitor-free tubes, and those for research use only. Essentially, the major change to the PCR machine is the number of slots available to add samples. The packaging of the test tubes should remain the same and can be purchased from the company mentioned above. Additionally, one can buy well-plates, strip-tubes, caps and seals, single tubes, and adhesive films for the any research or to accompany the sample tubes for the PCR machine. Feature 2: Hardware - PCR Machine & Fluorimeter Fluorimeters usually contain two beams that work together to decrease the noise created by power fluctuations. The upper beam passes through the sample while the second and lower beam tries to match the fluorescent power given off by the sample. The fluorimeter in our system did not change as our focus was on the PCR machine and how to improve it. One of the weaknesses we addressed was that the PCR takes too much time to analyze 16 samples. We fixed this problem by adding more slots to the PCR machine. With more slots, more samples were able to be analyzed in a the same amount of time. Instead of 16 slots, we created a PCR machine that contains 25 slots. This way, a researcher would not have to wait until one cycle was over to replace the samples with new ones and obtain the results.