BYU iGEM/Notebook/9 February 2011

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Multiple Cloning Site Addresses

Updated idea of our multiple cloning sites.

Part Placement within MCS
Part Placement within MCS


  • Made small primers (F & R) for GFP with restriction sites for Pbad
  • Made long primers (F & R) for five variations of our ΔTRS.
PDF with primer sequences
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