Barnes:Protocols

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Lab Protocols Experimental protocols developed and used within the Barnes Lab: Cell and Molecular Biology: Agarose gel electrophoresis protocol:    File:Gel Electrophoresis.pdf Electroporation protocol:    File:Electroporation.pdf Preparing electrocompetent cells:    File:Electrocompetent Cells.pdf Mini-prep protocol:    File:Protocol- Mini-Prep.pdf Preparing glycerol stocks:    File:Glycerol Stocks.pdf Equipment instructions: Operating instructions for flow-cytometer:    File:Flow-SOP.pdf Operating instructions for syphonstat:    File:Syphonstat.pdf Other: Solvent extraction of SCFAs:    File:Protocol-Solvent Extraction SCFAs.pdf Egg prep for C.elegans:    File:Protocol- Egg Prep.pdf