Kostov Y., Harms P., Randers-Eichhorn L., Rao G. (2001) Low-cost microbioreactor for high-throughput bioprocessing. Biotechnology and Bioengineering, 72, 346-352. Winner of Gaden Award for most Influential paper in Biotechnology and Bioengineering for 2001
The design of a microbioreactor is described. An optical sensing system was used for continuous measurements of pH, dissolved oxygen, and optical density in a 2 mL working volume. The KLa of the microbioreactor was evaluated under different conditions. An Escherichia coli fermentation in both the microbioreactor and a standard 1 L bioreactor showed similar pH, dissolved oxygen, and optical density profiles. The low cost of the microbioreactor, detection system, and the small volume of the fermentation broth provide a basis for development of a multiple-bioreactor system for high-throughput bioprocess optimization. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 346–352, 2001.
Keywords: microbioreactor; optical sensing; fermentation