CDNA synthesis

• Based on UltraScript™ cDNA Synthesis Kit [[[1]]]
• Using different kit will need changes in the protocol.

Reaction setup

1. Allow 5x cDNA Synthesis Mix to thaw, briefly vortex.
2. Prepare a master mix based on the following table [[[2]]]. Insert reagents in sequence listed.
3. Preper mix for No RT control setup (optional) acurding to the table [[[3]]] *helps in identifing DNA contamination.

excel sheet for sample amount calculations [[[4]]]

Incubation and enzyme denaturation(in the PCR)

1. Incubate at 42 °C for 30 minutes
2. Incubate at 85 °C for 10 minutes to denature RTase