Crisanti:Andrew M Hammond
Andrew M Hammond
Division of Cell & Molecular Biology
South Kensington Campus, SAF
- 2012 - Present, PhD, Imperial College London
- 2011, BSc, Imperial College London
I am a PhD student in Crisanti Lab at Imperial College, working towards the development of a synthetic gene drive strategy for population suppression in malaria mosquitoes . As a lab, we are investigating the use of HEGs, TALENs and CRISPR as a homing endonuclease to invade and subsequently supress mosquito populations – as initially theorised by the principal investigator, Professor Austin Burt , . Take a look at some of our most recent developments here ,,,.
My work is focused upon CRISPR, validating it as a tool for genome engineering in mosquitoes and testing the potential to use it in a gene drive. Previous research has predicted that an endonuclease-based gene drive targeting a female fertility gene would efficiently suppress populations if the mutations to this gene result in a recessive sterile phenotype .
We have made great steps towards achieving this ambitious aim, including the first use of CRISPR in Anopheles gambiae mosquitoes. To date, we have used CRISPR to characterise in vivo a number of recessive female sterile genes - demonstrating a high level of CRISPR activity in the process. Early results suggest that we can precisely engineer the genome of mosquitoes using CRISPR and that the technology meets all of the requirements for use a population suppressor.
- Hammond, A. M. & Nolan, T. (2014). Sex-, tissue- and stage-specific transgene expression. In: Benedict, M. (Ed.), Transgenic insects: techniques and applications (pp. 29-50). Oxfordshire, UK: CABI. isbn=978-1-78064-451-6