DIYbio:Notebook/Open Gel Box 2.0/Power Supply/Typical Setup
- Typical Power Supply Setup in NW Lab
- 1.2% gel is good for 100bp - 5kb (but by experience I would run 2% gel for 100-500bp)
- The run time for 96v at 84mA for a 1000bp (see reference for typical prokaryote gene size I currently work with) band takes about 20 minutes to resolve, up to 40 minutes to get a good separation if you have multiple near-by bands.
- 60V gets very good separation and clean looking 1kb bands, 120V runs fast but doesn't come out as well.
Select Typical Agarose Gel Concentrations†
|Agaros Conc.||Resolution Range||Run Length|
|0.8%||800 bp – 10 kb||5.8 cm|
|1%||400 bp – 10 kb||3.2 cm|
|1.2%||100 bp – 5 kb||5.8 cm|
|2%||100 bp – 2 kb||5.8 cm|
|4%||20 bp – 500 bp||5.8 cm|
† Invitrogen http://tools.invitrogen.com/content.cfm?pageid=11229