Dandekar & Chandler: Colony PCR for non-E. coli strains

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A pseudomonas colony PCR method that seems to work

  • Scoop up a grain of rice-sized blob of cells (use a stick, blue loops are too big to fit to the bottom of eppi tube)
  • Resuspend in 50 ul sterile dH20
  • Boil for 5 min at 100°C - caps may pop open, so use the fasteners or watch for swamping by dirty water
  • Spin down tubes at 16000g for 5 min
  • Use 5 ul of supernatant in PCR reaction
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