Davidying: SAGA Subunits

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SAGA Protein Subunit Descriptions

SAGA subunits, S. cerevisiae

Ada subunits size,chromosome,null p-type notes
Ada1 (aka HFI1, SUP110, SRM12, GAN1) 1.467 kb/489 aa, Chr. XVI,
viable
adds structural integrity to SAGA Complex, when deleted mutants show reduced fitness in rich media and decreased metabolite accumulation, growth defect with non-fermentable carbon source
Ada2 (aka SWI8) 1.305 kb/434aa, Chr. IV,
viable
transcription coactivator, when deleted mutants show reduced fitness in rich media, increased metabolite accumulation (glycogen), decreased drug resistance
Ada3(aka NGG1, SWI7) 2.109 kb/702aa, Chr. IV,
viable
transcriptional regulator, decreased drug resistance, reduced fitness in rich media
Gcn5 (aka ADA4, SWI9) 1.32 kb/439aa, Chr. VII,
viable
Histone acetyltransferase, catalytic subunit of ADA and SAGA, when deleted showed reduced fitness in rich media
Ada5 (aka SPT20) 1.815 kb/604aa, Chr. XV,
viable
subunit of SAGA transcriptional regulatory complex, adds structural integrity, when deleted mutants show decreased drug resistance and and decreased metabolite accumulation
Spt subunits size, chromosome, null p-type notes
Spt3 1.014 kb/337aa, Chr. IV,
viable
interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters, when deleted show reduced fitness in rich media, show decreased resistance to wortmannin and increased resistance to rapamycin, decreased metabolite accumulation
Spt7(aka GIT2) 3.999 kb/1332aa, Chr. II,
viable
involved in proper assembly of SAGA complex, increased drug resistance to rapamycin, decreased to wortmannin
Spt8 1.809 kb/602aa, Chr. XII,
viable
required for SAGA-mediated inhibition at some promoters, same thing about drug resistance, decreased metabolite accumulation
Spt20 (aka Ada5) 1.815 kb/604aa, Chr. XV,
viable
adds structural integrity to SAGA Complex, same thing about drug resistance, decreased metabolite accumulation
TAF subunits size, chromosome, null p-type notes
TAF5 (aka TAF90) 2.397 kb/798aa, Chr. II, inviable involved in RNA polymerase II transcription initiation and in chromatin modification, dead
TAF6 (aka TAF60) 1.551 kb/516aa, Chr. VII, inviable see above
TAF9 (aka TAF17) 0.474 kb/157aa, Chr. XIII, inviable see above
TAF10 (aka TAF23, TAF25) 0.621 kb/206aa, Chr. IV, inviable see above
TAF12(aka TAF61, TAF68) 1.620 kb/539aa, Chr. IV, inviable see above
Tra1 subunit size, chromosome, null p-type notes
Tra1 11.235 kb/3744aa, Chr. VIII, inviable interacts with acidic activators to initiate transcription, dead
other subunits size, chromosome, null p-type notes
Sgf73 1.974 kb/657aa, Chr. VII ,
viable
involved in formation of the preinitiation complex assembly at promoters, decreased metabolite accumulation, reduced fitness in rich media
Sgf29 0.779 kb/259aa, Chr. III,
viable
function unknown, pH sensitive after 8 generations, reduced fitness in rich media
Sgf11 0.3 kb/99aa, Chr.XVI,
viable
regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation, when deleted showed reduced fitness in rich media
Ubp8 1.416 kb/471aa, Chr. XIII,
viable
Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B, reduced fitness in rich media
Sus1 gene with intron, Chr. II,
viable
Protein involved in mRNA export coupled transcription activation
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