Dropbase: AADS sorter

Home        Devices        Contact       

Overview

Description

• Device for sorting 200 pL droplets. They fit perfectly on the tip of the cone before joining the spacing oil channels
• The height of the channels was 50 µm
• The droplets get slightly squeezed at the exact location of absorbance reading
• The wall between the sorted and waste channels contains apertures to allow oil flowing between the channels, reducing backpressure
• This flow of oil also allows to move droplet satellites back to the waste channel while keeping the sorted droplets in the good channel

Reference

Ladeveze et al, Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets, ACS Catal, 2023, doi:0.1021/acscatal.3c01609

Downloads

AADS with backpressure stabilizer: png, dwg

Usage Notes

• When using a spacing oil flow of 25-35 µL/min with a reinjection of droplets at 2-3 µL/min, we reached a sorting frequency up to 200Hz . SL