Dropbase: Double-emulsion-02
Overview
Description
• Devices to generate 7 pL double emulsions (~ 20µm) or Water-Oil-Water droplets for analysis by Flow Cytometry or sorting by FACS
• The first chip has 3 inlets for (i) Cells (ii)Substrate and (iii) the carrier oil+ surfactant phase
• The second chip has 2 inlets for (i) the first emulsion and (ii) a carrier aqueous phase.
Reference
Wong CFA, van Vliet L, Bhujbal SV, Guo C, Sletmoen M, Stokke BT, Hollfelder F, Lale R. A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. ACS Synthetic Biology (2021). https://doi.org/10.1021/acssynbio.1c00084
Designed by: Tomasz Kaminski, Remkes Scheele and Yanik Weber.
Downloads
Usage Notes
Please enter any comments that other users may find useful below this note (such as flow rates that worked well for particular oil/aqueous phases). When providing usage notes please provide as much detail as useful. We would request that you 'sign' any comments with your initials.
The second chip should be rendered hydrophilic following the procedure detailed in the publication.
First chip: flow rates were 1 μL/min total for the aqueous phases (cells and substrate) and 8 μL/min for the carrier oil phase.
Second chip: start with 0.5µL/ min for the emulsion and 1µL/min for the aqueous phase, ensuring that the first emulsion is well packed. LvV, 2021
