Dropbase: UHT-AADS Sorter
Overview
Description
The UHT-AADS showing the single 50 µm layer channel (light blue) and a close-up design of the sorting junction showing the gapped divider at the bifurcation (light blue). The gapped divider is on the second 50 µm layer. 1) input channel for droplets, 2) input channel for spacing oil, 3) input channel for bias oil, 4) positive channel outlet, 5) negative channel outlet, 6). The ground electrode is coloured black and the positive electrode is coloured red. Adding a single-layer droplet injection chamber allows for even spacing of the 75 pL droplets. The bias oil channel acts as a barrier to droplets from entering the negative chamber unless acted on by the dielectrophoretic force.
Reference
E. J. Medcalf et al. 'Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies', bioRxiv. 2022,(https://www.biorxiv.org)
Designed by: Elliot J. Medcalf and Tomasz Kaminski
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