ESSCOSMOS/2009:Enzymes Soil & Fungal Enzymes
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Overview
In this activity, we will study the organisms and environment along a gradient from the edge of a marsh to the top of the hill.
Objectives
- Investigate the relationship between enzyme activity and CO2 release from substrate being decomposed by different species of mushrooms.
- Create graphical summaries of the findings
- Enter data into class database
Background
Experimental protocol
We will be using a colorometric method developed by Dr. Steve Allison to measure the activity of enzymes in substrate that it colonized by mushroom mycelia.
We will follow the methods outlined here: experimental protocol