Eccles:new lab member orientation

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Eccles Lab > General Lab info

Contents

Welcome to the Developmental Genetics Group (DGG).

Addresses

Our postal address is;

Developmental Genetics Group
Department of Pathology
Dunedin School of Medicine
University of Otago
PO Box 913
DUNEDIN 9054


Our delivery address is;

Developmental Genetics Group
Department of Pathology
Hercus Building
cnr Great King and Hanover Streets
Dunedin School of Medicine
University of Otago
DUNEDIN

Fax Number

(housed in Hercus G11)
479 7136 (local)
03 479 7136 (national)
64 3 479 7136 (international)


DGG Locations.

Offices.
Hercus 201a: Professor Miichael Eccles, DGG director
Hercus 203a: Lucy Gray, departmental research administrator
Hercus 207: Dr Cherie Stayner, PKD Group principal investigator
(within 206): Dr Aaron Jeffs, programme grant principal investigator
Hercus 230: Dr Adele Woolley, programme grant research fellow
Hercus 230: Dr Shujie He, programme grant principal investigator
Hercus 232: Grace Li (PhD student
Hercus 232: Nicki Sheratt (MSc student)
Scott 201: Michael Algie (Assistant Research Fellow)
Scott 201: Lana Ellis (Assistant Research Fellow)
Scott 201: Amy Glover (Assistant Research Fellow)
Scott 201: Matthew Hamilton (Technical Officer)
Scott 201: Jody Hazlett (Assistant Research Fellow)
Scott 201: Jane Wilson (PhD student)

Labs.
Hercus 206: PKD Group
Hercus 210: Programme grant staff and students
Hercus 203: Programme grant staff and students
Hercus 202: Shared Cell Culture facility (2nd floor)
Hercus 212: Programme grant staff and students

Key Departmental People.

  1. Lynne Hananeia - Purchasing Administrator and Health and Safety Officer
    Hercus G03a
    extension 7276
    lynne.hananeia@stonebow.otago.ac.nz
    path.purchasing@stonebow.otago.ac.nz for all purchasing
    See Lynne if you have any health and safety issues, concerns, questions.
    All external ordering goes through Lynne (see section on ordering)
  2. TBA - Department Manager and Head Technician
    Hercus G02
    extension 7153
    Until a new Department manager is appointed see Lynne Hananeia or Lucy Gray for;
    *Keys
    *Form for getting staff ID card and after hours access
    *Form for income direction
    Until a new Department manager is appointed see Lynne Hananeia regarding any technical issues in the department such as;
    *broken departmental equipment (eg: autoclaves)
    *leaks etc
    Once you have your staff ID card let Lynne Hananeia know your name and staff number to gain swipe card access to the research laboratory areas
  3. Pam Baxter - Departmental Secretary
    Hercus G11
    extension 7142
    Once you have your staff ID see Pam for getting a staff email account set up.


General Lab Information

Laundry

All clean laundry (labcoats, towels and red lab teatowels) is housed in the cupboard in Hercus 215.
Dirty laundry goes in the yellow cloth bag on hook in Hercus 215.
Towels should not be used to mop up chemical or biohazardous spills. Use designated chemical and biohazardous spill kits and paper towels for this.

Biohazardous Waste

We have numerous biohazard bins in our labs. It is everyone's responsibility to empty full rubbish bags and provide clean ones. Full biohazard bags are taped closed and are taken to the collection cage on the ground floor of the Hercus building in the loading bay. Use the goods lift accessed via Hercus 215 to transport biohazard bins and bags. Your key works the collection cage padlock. Clean biohazard bags in labelled drawer in lab 206 and lab 203 to fit the wheely bins. Smaller bags are available in lab 210 and lab 202 to fit the normal rubbish bins. If there are no bags left in the laboratory, more bags can be found in Hercus G01.
New sharps and pipette disposal buckets can also be found in Hercus G01. Full buckets are disposed of on the top shelf of the collection cage.

Autoclaves

There are two departmental autoclaves. One in room 215a second floor and one in Hercus 117 on first floor. Please make sure you ask an experienced person to show you how to use the autoclaves safely and effectively before using on your own.

Ice

There are two departmental ice machines, one on each research lab floor. One is housed in Hercus 117, first floor. It is on the LHS as you walk into the room, right beside the hand basin. The second is housed in Hercus 215a.

Milli Q Water

This machine is in Hercus 117. On LHS wall above stainless steel bench and sink.
Push “operate/standby” button to operate.
Once pressure reaches 18.2 turn dial from recirculate to production
Let a bit of milli Q water flow out before collecting what you need.
Milli Q water is sterile, RNase and DNase free.
It is not necessary to autoclave before using.
Suitable for PCR.
However regarding RNA work, it may be worth DEPC treating this water as the outlet hose which dangles into the sink is probably not RNase free.
Once you have the volume you need turn the dial back to recirculate to stop the water flow.
Push “operate/standby” button to leave in standby mode.

Distilled Water

Each lab has a dH2O hose above the sink. Autoclave if desired.

pH Metres

In lab 206 on shelf behind fine balance. Portable and very easy to use. Also have access to Stephen Robertson's pH metre.

Balances

Fine balance in lab 206, lab 212. For weighing agarose and other larger weights please use Stephen Roberson's balances.


Useful internal web links (accessible directly on-campus, or via staff/student proxy off-campus)

  1. http://genedb.otago.ac.nz/cogs
    Record of all product orders generated by our group (initially set-up to track HRC grants). See Aaron for access to this link.
  2. http://genedb.otago.ac.nz/btc/
    This link was set up as a way of facilitating communication within the HRC-funded programme grant for which Antony Braithwaite, Janice Royds and Michael Eccles are all principal investigators. This link is useful as a calendar for letting others know when you are going to be away. It is also useful for finding out what seminars are coming up. All members of the DGG use this link for these purposes (you don’t need to be a programme grant employee).

Equipment booking

  1. Book 7300 qPCR machine, Axon array scanner, and Bioanalyzer here. Login required, see Aaron for access.
  1. Book old 7000 qPCR machine here. Login required.
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