Elizabeth Polidan Week14

From OpenWetWare
Jump to navigationJump to search
My children

Elizabeth Polidan

BIOL 398.03 / MATH 388

  • Loyola Marymount University
  • Los Angeles, CA, USA

Elizabeth Polidan Home

Course Home


Week 14 Assignment

Laboratory Notebook

  1. Copied sample data file, full_schade_data.xls, to Model_Input_Polidan.xls.
  2. Copied the transposed regulation matrix generated in YEASTRACT with the following changes:
    • Modified names across the top row to use same format as is used in the columns (e.g. CIN5, not Cin5p).
    • Alphabetized transcription factor names.
  3. Placed modified transposed regulation matrix into three worksheets in Model_Input_Polidan.xls:
    • network
    • network_weights
    • network_thresholds
  4. Created two columns of transcription factor names using list from matrix in step 2.
    • Obtained systematic names from YEASTRACT.
  5. Edited the worksheet called "degradation_rates".
    • Pasted the two columns from step 4.
    • Looked up the degradation rates in a paper by Belle et al. (2006) and added them to worksheet. Used the value "0.027182242" if the degradation rate was unavailable for a transcription factor.
  6. Edited worksheet called "production_rates".
    • Pasted the two columns from step 4.
    • Created equations that multiplied referenced production rates by 2.
  7. Edited worksheet called "log2_concentrations".
    • Pasted the two columns from step 4.
    • Copied the values for Average Log Fold Change for times 15, 30, and 60 minutes from the file, Polidan_wildtype_data_20130402_full.xls, from Assignment 9.
  8. Edited worksheet called "concentration_sigmas".
    • Pasted the two columns from step 4.
    • Calculated standard deviation of Log Fold Change for times 15, 30, and 60 minutes from the file, Polidan_wildtype_data_20130402_full.xls, from Assignment 9. Used same columns that were used in calculating the mean Log Fold Change.
    • Pasted the values into the concentration_sigmas worksheet.
  9. Left the "optimization_parameters" worksheet unchanged.
  10. Edited worksheet called "simulation_times". Changed times to 0, 5, 10, 15, … 60.
  11. Edited worksheet called "network_b".
    • Pasted the first column from step 4 – this sheet does not have the systematic names.
    • Ensured the "threshold" value for each gene is "0.000".
  12. Upload file to LionShare and sent and email to Drs. Dahlquist and Fitzpatrick with a link to the file.