Endy:F2620/Standard chassis preparation
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Wherever possible, we prepare chassis bearing T9002 using the standard method described below. This insures uniformity of conditions between different experiments and repeats.
- Inoculate 5ml of Supplemented M9 medium and antibiotic (kanamycin, 20μg/ml) with a single colony from a fresh plate. Grow the culture in a 17mm test tube for 15hrs at 37C with shaking at 70rpm.
- Dilute the culture 1:1000 into 5ml of fresh medium and grow for an additional 5 hours under the same conditions.