Endy:Screening plasmid/v2.0/Construction/Queue

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This page is to keep up with the constructions that are in progress. When a part is completed be sure to add it to the glycerol box. To find out what these parts are please read testing or construction areas.

Contents

Todo list

To be designed

Completed (seq-verified)

  • <bbpart>I13545</bbpart> (GFP.ins_site.mRFP1)
  • <bbpart>I13546</bbpart> (F2620 + GFP.ins.mRFP1) -- 1360-1498 (missing ~10 bp in here)
    • between 1350 and 1450 there are 11 N’s. accoding to the chromatogram each one is most likely correct.
  • <bbpart>I13547</bbpart> (F2621 + GFP.ins.mRFP1)
    • 14 N’s between 1440 and 1510. Most of them checked out OK, some ambiguous.
  • <bbpart>I13549</bbpart> (F2620 + mRFP1)
  • <bbpart>I13550</bbpart> (F2621 + mRFP1)
  • <bbpart>I13551</bbpart> (F2620 + ins.mRFP1)
  • <bbpart>I13552</bbpart> (F2621 + ins.mRFP1)
  • <bbpart>I13553</bbpart> (PenI + GFP/RFP)
  • <bbpart>I13554</bbpart> (F2620 + GFP)
  • <bbpart>I13555</bbpart> (F2621 + GFP)
  • <bbpart>I13558</bbpart> (Ptet expressing G/hairpin/RFP)
  • <bbpart>I13559</bbpart> (F2620.CFP)-- middle unsequenced

Awaiting sequencing results

Length-verified

  • <bbpart>I13561</bbpart> - looks (much) too short, checking sequence
  • <bbpart>I13562</bbpart> - looks (a little) too short, checking sequence

To be digested

To be prepped

Planning

  • <bbpart>I13556</bbpart> - F2620 + mCherry
  • <bbpart>I13557</bbpart> - F2621 + mCherry
  • <bbpart>I13560</bbpart> - F2621 + CFP
  • <bbpart>I13562</bbpart> - polycistronic mCherry/GFP
  • <bbpart>I13561</bbpart> - polycistronic mRFP1/GFP
    • The above two don't contain BB sites, they are just control constructs

To move into MG1655

  • <bbpart>I13546</bbpart> - F2620+GFP/RFP (need to prep more dna)
  • <bbpart>I13547</bbpart> - F2621+GFP/RFP (need ot prep more dna)
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