Haynes Lab:Notebook/CRISPR Editing/2014/10/30

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10/30/2014

Picked colonies from transformation plates for gRNAs 26, 30, 39-49.

Minipreps for g033, g037, g038. Tested whether double eluting helps. I see these differences when reading the same sample twice. Can't conclude it makes any significant difference.

Sample Read# ' 260 280 260/280 ng/┬ÁL
g037 in pX330single elution0.0250.0141.78925.032
double elution0.0290.0161.80728.52
g033 in pX330single elution0.1620.0851.907162.222
double elution0.1640.0861.91164.041
g038 in pX330single elution0.0280.0141.96327.505
double elution0.0310.0171.87330.949



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