05/20/2015
We mini-prepped receivers and synthases for gel verification.
Sample Read#
|
260
|
280
|
260/280
|
ng/µL
|
AubR 1 |
0.711 |
0.371 |
1.914 |
711.002
|
AubR 2 |
0.628 |
0.333 |
1.885 |
628.208
|
BraR 1 |
0.824 |
0.433 |
1.904 |
824.021
|
BraR 2 |
0.485 |
0.255 |
1.906 |
485.463
|
RpaR 1 |
0.705 |
0.369 |
1.91 |
705.115
|
RpaR 2 |
0.572 |
0.304 |
1.886 |
572.435
|
AubI 1 |
0.399 |
0.21 |
1.902 |
399.31
|
AubI 2 |
0.563 |
0.313 |
1.8 |
563.178
|
AubI 3 |
0.567 |
0.299 |
1.895 |
567.352
|
BjaI 1 |
0.577 |
0.304 |
1.898 |
577.174
|
BjaI 2 |
0.561 |
0.292 |
1.921 |
561.178
|
BjaI 3 |
0.589 |
0.312 |
1.891 |
589.038
|
Sample Read#
|
260
|
280
|
260/280
|
ng/µL
|
BraI 1 |
0.542 |
0.281 |
1.933 |
542.456
|
BraI 2 |
0.59 |
0.306 |
1.926 |
589.609
|
CerI 1 |
0.638 |
0.333 |
1.916 |
637.778
|
CerI 2 |
0.789 |
0.413 |
1.911 |
789.27
|
EsaI 1 |
0.573 |
0.298 |
1.922 |
572.722
|
EsaI 2 |
0.216 |
0.114 |
1.899 |
215.855
|
LasI 1 |
0.373 |
0.195 |
1.915 |
373.17
|
LasI 2 |
0.172 |
0.089 |
1.923 |
171.718
|
RpaI 1 |
0.406 |
0.214 |
1.899 |
405.978
|
RpaI 2 |
0.646 |
0.336 |
1.921 |
646.406
|
SinI 1 |
0.798 |
0.42 |
1.898 |
797.594
|
SinI 2 |
0.691 |
0.364 |
1.9 |
691.117
|
The synthases were double digested with EcoRI and XbaI; Receivers were digested with KpnI and EcoRI. Here is a gel of the digestion reactions:
Based on the gel, we think the following colonies worked: BraI 2, CerI (1?), 2, EsaI 2, RpaI1 for the synthases. [ ] for the receivers. (all?). Ryan will send them in for sequencing to verify. Meanwhile, we will pick more colonies from the ones that didn't work and repeat the process.
|