ICSynBio:27 01 20WL

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Wet Lab Notebook

Week of 27/01/20

• Collection of Parts in Benchling

Week of 03/02/20

Planning session for the wet lab process. Stages to the wet lab process are:

• Gathering Parts
• Creating Parts
• Buying Parts
• Molecular Cloning Assembly Protocol (MoClo)
• Insertion into Plasmid
• Transformation into e.coli
• Cultivating Culture
• Blue-White Screening
• Miniprep isolation of plasmid
• Restriction Enzyme Digestion
• Gel Electrophoresis
• UV Transillumination
• Toggle Switch Testing
  • IPTG Induction
  • aTc Induction
  • Heat Induction
• Flow Cytometry

Week of 10/02/20

Olivia and Sam learn the following techniques:

• Making and Pouring Agarose Gel
• Streaking Bacterial Culture on Agarose Gel
• Pipetting Techniques

Week of 17/02/20

17/02/20

• Streaking the DVs and the two control plasmids which contain GFP and RFP.
• Cultures grown on 14/2 are okay except one didn’t have a lot of colonies.

19/02/20

• Miniprep isolation of plasmid
• Cultures grown on 17/2 is okay