IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/05/15

iGEM Project name 1

Main project page Previous entry      Next entry

Competent Cell Preparation (for each strain) Discovered that the shaking incubator had crept up to 37°C overnight. Diluted 0.45mL of culture into 50mL LB medium. Incubated at 30°C in shaking incubator, starting at 8:50AM. Checked OD600nm at 2:50PM: DH5a 0.6 0.8 (used as 0.7) DB3.1 1.8 1.3 (used as 1.8) --> diluted to 0.4 in 40mL LB. Followed competent cell protocol exactly as written. Used non-sterile centrifuge bottles, so there is some risk of contamination. Stored cells at 4°C at 6:36PM.