IGEM:Cambridge/2008/Notebook/Voltage/2008/07/30

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Competence test Transformed PUK plasmid and YFP plasmid into mutant E.coli strains. osmY and Kdp digested PCR products, and death gene vector with EcoR1 and Pst1 ligated new BioBricks into vector Transformed into competent TOP10 cells Plate out onto Chloramphenicol plates