IGEM:Groningen/Notebook/iGEM 2011/2011/08/15

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15-8-11 colony PCR of taRNA-pSB1A3-DT: Composition master mix: 10× Taq buffer: 40μl dNTPs 10mM: 8μl MgCl2: 24μl Forward biobrickvector primer: 8μl Reverse biobrickvector primer: 8μl Taq polymerase: 2μl MQ water: 310μl PCR conditions: Pre heated lid: 111°C Denaturation: 94°C for 10min Cycle (33×) Denaturation: 94°C for 30s Annealing: 60°C for 30s Extenstion: 72°C for 30s Final extension: 72°C for 10min Store infinite at 4°C Analyse PCR samples on a 1% agarosegel Check sequencing results PBAD-RBS-GFP-DT again the GFP part does not look well Filling in 'stageformulieren' Grow TOP10 competent cells in M9 minimal medium and also E.coliDH5alpha, to see tomorrow if the TOP10cells grow better compared to the E.coli DH5alpha cells... Cleaning up the lab for tomorrow's audit!