IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-31

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Frozen stocks and Miniprep of 10 KaiC + J04500 cultures grown up yesterday

I made frozen stocks and miniprepped the 10 cultures we inoculated yesterday: V2-4, V19-1, V19-4, V19-5, V19-6, V19-7, M2-7, M19-2, M19-4, M19-8.

Frozen stocks are located in the box simply labeled "cyano" in the -80C freezer.

The plates we streaked yesterday are in our 4C fridge.

Sequencing of 10 KaiC + J04500 cultures

I sent off 20 sequencing orders (VF2 and VR primers for each of the 10 cultures). I did not rush the order, so it probably won't be completed until next Monday or Tuesday.

Name Plasmid Primers
V2-4-FV2 #4VF2
V2-4-RV2 #4VR
V19-1-FV19 #1VF2
V19-1-RV19 #1VR
V19-4-FV19 #4VF2
V19-4-RV19 #4VR
V19-5-FV19 #5VF2
V19-5-RV19 #5VR
V19-6-FV19 #6VF2
V19-6-RV19 #6VR
V19-7-FV19 #7VF2
V19-7-RV19 #7VR
M2-7-FM2 #7VF2
M2-7-RM2 #7VR
M19-2-FM19 #2VF2
M19-2-RM19 #2VR
M19-4-FM19 #4VF2
M19-4-RM19 #4VR
M19-8-FM19 #8VF2
M19-8-RM19 #8VR
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