IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-31
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Frozen stocks and Miniprep of 10 KaiC + J04500 cultures grown up yesterday
I made frozen stocks and miniprepped the 10 cultures we inoculated yesterday: V2-4, V19-1, V19-4, V19-5, V19-6, V19-7, M2-7, M19-2, M19-4, M19-8.
Frozen stocks are located in the box simply labeled "cyano" in the -80C freezer.
The plates we streaked yesterday are in our 4C fridge.
Sequencing of 10 KaiC + J04500 cultures
I sent off 20 sequencing orders (VF2 and VR primers for each of the 10 cultures). I did not rush the order, so it probably won't be completed until next Monday or Tuesday.
Name | Plasmid | Primers |
V2-4-F | V2 #4 | VF2 |
V2-4-R | V2 #4 | VR |
V19-1-F | V19 #1 | VF2 |
V19-1-R | V19 #1 | VR |
V19-4-F | V19 #4 | VF2 |
V19-4-R | V19 #4 | VR |
V19-5-F | V19 #5 | VF2 |
V19-5-R | V19 #5 | VR |
V19-6-F | V19 #6 | VF2 |
V19-6-R | V19 #6 | VR |
V19-7-F | V19 #7 | VF2 |
V19-7-R | V19 #7 | VR |
M2-7-F | M2 #7 | VF2 |
M2-7-R | M2 #7 | VR |
M19-2-F | M19 #2 | VF2 |
M19-2-R | M19 #2 | VR |
M19-4-F | M19 #4 | VF2 |
M19-4-R | M19 #4 | VR |
M19-8-F | M19 #8 | VF2 |
M19-8-R | M19 #8 | VR |