IGEM:Harvard/2006/Fusion proteins/Oligos
Streptavidin primers
StrepF, forward primer for SA, no start codon
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [GCTGAAGCTGGTATCACCGG] 3'
StrepR, reverse primer for SA, no stop codon
- 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [GGAAGCAGCGGACGGTTTAA] 3'
StrepFS, forward primer for SA w/ start codon
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [ATGGCTGAAGCTGGTATCAC] 3'
StrepRS, reverse primer for SA w/ two stop codons
- 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [TTATTAGGAAGCAGCGGACG] 3'
StrepMF, forward primer for mutagenesis removing XbaI site in SA
- 5’ CTGAAAGCCGCTACGTTCTGACCGGTCGT 3’
StrepMR, reverse primer for mutagenesis removing XbaI site in SA
- 5’ ACGTAGCGGCTTTCAGCGTTACCAACAGC 3’
StrepMF2, alternate forward primer for mutagenesis removing XbaI site in SA
- 5' CTGAATCGAGATACGTTCTGACCGGTCGT 3'
StrepMR2, alternate reverse primer for mutagenesis removing XbaI site in SA
- 5' ACGTATCTCGATTCAGCGTTACCAACAGC 3'
StrepHR, reverse primer for SA+His6tag, no stop codon
- 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [ATGGTGGTGATGGTGATGGG] 3'
StrepHRS, reverse primer for SA+His6tag w/ two stop codons
- 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [TTATTAATGGTGGTGATGGT] 3'
OmpA primers
OmpAF, forward primer for full OmpA
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA G [atgaaaaaga cagctatcgc] 3'
OmpAR, reverse primer for full OmpA
- 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT A [ttaagcctgc ggctgagtta] 3'
OmpA46F, forward primer for OmpA starting aa 46
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [aacccgtatgttggctttga] 3’
OmpA66R, reverse primer for OmpA ending aa 66
- 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [GCTGCCTTTGTACGGCATAC] 3’
OmpA159R, reverse primer for OmpA ending aa 159
- 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [GTTGTCCGGACGAGTGCCGA] 3’
Lpp primers
LppF, forward primer for Lpp
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [atgaaagctactaaactggt] 3’
Lpp29R, reverse primer for Lpp ending aa 29
- 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [CTGATCGATTTTAGCGTTGC] 3’
Lpp78R, reverse primer for Lpp ending aa 78
- 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [CTTGCGGTATTTAGTAGCCA] 3’
Lpp78RS reverse primer for Lpp ending aa 78 and stop codon
- 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [TTACTTGCGGTATTTAGTAG] 3’