IGEM:Harvard/2006/vlau/Notebook/2006-7-19

Pre-Working Stocks & Working Stocks

1. Rxn Mixtures

Description	Plate Locations	Total
c4.0.1: core barrel oligos	box 4_A: row A (1-12), row B (1-12), row C (1-12), row D (1-12), row E (1-12), row F (1-6)	66
c4.0.2: core top lid oligos	box 4_A: row F (7-12), row G (1-12), row H (1-12); box 4_B: row A (1-3)	33
c4.0.3: core bottom lid oligos	box 4_B: row A (4-12), row B (1-12), row C (1-12)	33
c4.0.4: barrel oligos @ outside aptamers -aptamers	box 4_B: row D (1-4)	4
c4.0.5: barrel oligos @ outside aptamers +aptamers	box 4_B: row D (5-8)	4
c4.0.6: barrel oligos @ inside aptamers -aptamers	box 4_B: row D (9-12)	4
c4.0.7: barrel oligos @ inside aptamers +aptamers	box 4_B: row E (1-4)	4
c4.0.8: two-oligo latch sequences for barrel	box 4_B: row E (5-8)	4
c4.0.9: four-oligo latch sequences for barrel	box 4_B: row E (9-12)	4
c4.0.10: barrel oligos @ latches -latches	box 4_B: row F (1-4)	4
c4.0.11: two-oligo latch sequences for top lid	box 4_B: row F (5-6)	2
c4.0.12: two-oligo latch sequences for bottom lid	box 4_B: row F (7-8)	2
c4.0.13: four-oligo latch sequences for top lid	box 4_C: row A (1-2)	2
c4.0.14: four-oligo latch seequences for bottom lid	box 4_C: row A (3-4)	2
c4.0.15: top lid oligos @ latches -latches	box 4_C: row A (5-6)	2
c4.0.16: bottom lid oligos @ latches -latches	box 4_C: row A (7-8)	2
c4.0.17: two-oligo latch barrel displacement oligos	box 4_C: row A (9-12)	4
c4.0.18: two-oligo latch top lid displacement oligos	box 4_C: row B (1-2)	2
c4.0.19: two-oligo latch bottom lid displacement oligos	box 4_C: row B (3-4)	2
c4.0.20: four-oligo latch barrel displacement oligos	box 4_C: row B (5-8)	4
c4.0.21: four-oligo latch top lid displacement oligos	box 4_C: row B (9-10)	2
c4.0.22: four-oligo latch bottom lid displacement oligos	box 4_C: row B (11-12)	2
c4.0.23: barrel zipper oligos	box 4_C: row C (1-4)	4
c4.0.24: top lid zipper oligos	box 4_C: row C (5-6)	2
c4.0.25: bottom lid zipper oligos	box 4_C: row C (7-8)	2

Working Stocks	Description	Pre-Working Stocks	Water	Total
c4.0A	no latches, no aptamers	1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL)	39 μL	200 μL
c4.0B.core	latch design 1, no aptamers	1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL)	47 μL	200 μL
c4.0B.latches	latch design 1, no aptamers	8 (4 μL), 11 (2 μL), 12 (2 μL)	-	-
c4.0C.core	latch design 2, no aptamers	1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL)	47 μL	200 μL
c4.0C.latches	latch design 2, no aptamers	9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL)	-	-

2. Protocol

   - mixed 10 μL of each 50 μM stock oligo to make pre-working stocks
   - mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM