IGEM:Harvard/2006/vlau/Notebook/2006-7-31
From OpenWetWare
Jump to navigationJump to search
Rxn Mixtures
| Working Stocks | Description | Pre-Working Stocks | Water | Total |
| c3.2A | no latches, no aptamers | 1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 5 (3 μL), 8 (2 μL), 9 (0 μL) | 40 μL | 200 μL |
| c3.2H.core | latch design 2, in aptamers | 1 (94 μL), 2 (28 μL), 3 (30 μL), 5 (3 μL), 6 (3 μL) | 32 μL | 200 μL |
| c3.2H.latches | latch design 2, in aptamers | 10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL), 16 (4 μL) | - | - |
| c3.2I.core | latch design 2, out aptamers | 1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 7 (3 μL) | 32 μL | 200 μL |
| c3.2I.latches | latch design 2, out aptamers | 10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL), 16 (4 μL) | - | - |
| c4.0A | no latches, no aptamers | 1 (66 μL), 2 (33 μL), 3 (33 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL) | 52 μL | 200 μL |
| c4.0H.core | latch design 2, in aptamers | 1 (66 μL), 2 (33 μL)), 3 (33 μL)), 4 (4 μL), 7 (4 μL)) | 44 μL | 200 μL |
| c4.0H.latches | latch design 2, in aptamers | 9 (4 μL), 13 (2 μL)), 14 (2 μL)), 23 (4 μL)), 24 (2 μL)), 25 (2 μL)) | - | - |
| c4.0I.core | latch design 2, out aptamers | 1 (66 μL), 2 (33 μL), 3 (33 μL), 5 (4 μL), 6 (4 μL) | 44 μL | 200 μL |
| c4.0I.latches | latch design 2, out aptamers | 9 (4 μL), 13 (2 μL)), 14 (2 μL)), 23 (4 μL)), 24 (2 μL)), 25 (2 μL)) | - | - |
| Experiment | Scaffold | Oligos | Folding buffer | dH2O | total volume |
| c3.2.A | 9 μL p7308 | 16 μL (250 nM) | 4 μL 10x | 11 μL | 40 μL |
| c3.2H | 9 μL p7308 | 16 μL (250 nM) | 4 μL 10x | 11 μL | 40 μL |
| c3.2I | 9 μL p7308 | 16 μL (250 nM) | 4 μL 10x | 11 μL | 40 μL |
| c4.0A | 9 μL p7308 | 16 μL (250 nM) | 4 μL 10x | 11 μL | 40 μL |
| c4.0H | 9 μL p7308 | 16 μL (250 nM) | 4 μL 10x | 11 μL | 40 μL |
| c4.0I | 9 μL p7308 | 16 μL (250 nM) | 4 μL 10x | 11 μL | 40 μL |
| no scaffold | - | 16 μL (250 nM) | 4 μL 10x | 20 μL | 40 μL |
| no oligos | 9 μL p7308 | - | 4 μL 10x | 27 μL | 40 μL |
