IGEM:Harvard/2006/vlau/Notebook/2006-7-31

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Rxn Mixtures

Working Stocks Description Pre-Working Stocks Water Total
c3.2Ano latches, no aptamers1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 5 (3 μL), 8 (2 μL), 9 (0 μL)40 μL200 μL
c3.2H.corelatch design 2, in aptamers1 (94 μL), 2 (28 μL), 3 (30 μL), 5 (3 μL), 6 (3 μL)32 μL200 μL
c3.2H.latcheslatch design 2, in aptamers10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL), 16 (4 μL)--
c3.2I.corelatch design 2, out aptamers1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 7 (3 μL)32 μL200 μL
c3.2I.latcheslatch design 2, out aptamers10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL), 16 (4 μL)--
c4.0Ano latches, no aptamers1 (66 μL), 2 (33 μL), 3 (33 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL)52 μL200 μL
c4.0H.corelatch design 2, in aptamers1 (66 μL), 2 (33 μL)), 3 (33 μL)), 4 (4 μL), 7 (4 μL))44 μL200 μL
c4.0H.latcheslatch design 2, in aptamers9 (4 μL), 13 (2 μL)), 14 (2 μL)), 23 (4 μL)), 24 (2 μL)), 25 (2 μL))--
c4.0I.corelatch design 2, out aptamers1 (66 μL), 2 (33 μL), 3 (33 μL), 5 (4 μL), 6 (4 μL)44 μL200 μL
c4.0I.latcheslatch design 2, out aptamers9 (4 μL), 13 (2 μL)), 14 (2 μL)), 23 (4 μL)), 24 (2 μL)), 25 (2 μL))--


Experiment Scaffold Oligos Folding buffer dH2O total volume
c3.2.A9 μL p730816 μL (250 nM)4 μL 10x11 μL40 μL
c3.2H9 μL p730816 μL (250 nM)4 μL 10x11 μL40 μL
c3.2I9 μL p730816 μL (250 nM)4 μL 10x11 μL40 μL
c4.0A9 μL p730816 μL (250 nM)4 μL 10x11 μL40 μL
c4.0H9 μL p730816 μL (250 nM)4 μL 10x11 μL40 μL
c4.0I9 μL p730816 μL (250 nM)4 μL 10x11 μL40 μL
no scaffold-16 μL (250 nM)4 μL 10x20 μL40 μL
no oligos9 μL p7308-4 μL 10x27 μL40 μL
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