IGEM:IMPERIAL/2006/Protocols/20ul ligation

From OpenWetWare

Jump to: navigation, search

20μL Ligation

  • 14μL Insert (after glass milk purification)
  • 2μL 10x ligation buffer
  • 1μL Vector
  • 1μL T4 DNA ligase


  • Leave on bench for at least 1hr.
Personal tools