IGEM:IMPERIAL/2007/Calendar/2007-7-20

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We are in Week 2 of the iGEM project.


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Contents

Proposals

Task List

Students

Continue expanding list of new HRP literature
Continue characterization of HRP
Expand on contingency ideas (propose 3rd project)
Expand on HRP application ideas
Develop outlines of proposal presentations for Monday
Discuss proposal for database of experiments
Discuss considerations raised during Thursday's debriefing:

Considerations:

Glucose/Insulin System

  • Currently proposed receptor: for Glucose-6-P. Receptor available for glucose?
  • Nature of signal required to secrete insulin - pulse or other?


Thermogenic System

  • Will this plasma-based enzyme system lead to clumping (due to hydrophobicity) - efficiency issues
  • Look into alternative enzyme system which could be located intracellularly
  • Investigate actual thermogenic reaction - will the reaction catalyzed actually produce heat? Literature based?

Supervisors

Today's Schedule

Name Alex Anthony Ben Cheuk Dirk James Jerry Lucas Maira Peixuan
0900
0930
1000
1030
1100
1130
1200
1230
1300
1330
1400
1430
1500
1530
1600
1630
1700
1730
1800
Evening


Questions

Debriefing

Presentation/Discussion of Ideas


1. pH sensor (new)

Problems/Considerations

  • Effects of environmental pH on
    • cell growth
    • induction of Pcad
  • Time-scale of operation

Currently proposed system takes approximately 7 hours to reach steady-state. Look into faster/better pH sensing system

  • Possibility of incorporating a control system for pH


2. Thermosensing-Thermogenic system

Problems/Considerations

  • Check genebank for sequence of J45503?
  • Possibility for implementation of ATC?
  • Time scale. Propose different mechanism to accelerate this function
  • Incorporation of cold-shock element
    • Look into exploitable endothermic reactions
  • Range of operation (currently 30-37 degrees Celsius). Look into extension of range


3. Biofilms (new)

Problems/Considerations

  • Adhesion mechanics - pili, fimbrae, other?
  • Applications?


HRP Characterization

  • A indirect method for measuring PoPS in/out was proposed, using a GFP/RFP system. This was discussed with Frank.
  • The experiments, including equipment to be used, required were also discussed with Frank
  • Continue expanding on literature related to HRP
    • Research the strength of repression offerred by HrpV
    • Leakiness of promoters involved in HRP system?
    • Look into relevant parameters involved in characterization

Modelling

  • Look into available programs, including CellDesigner
  • Look into stochastic pi-calculus?
  • Start drawing model involved in HRP characterization, e.g. relation of PoPS IN/OUT to [GFP]/[RFP], etc.

Presentation Details

  • Details of presentations taking place on Monday were discussed
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