IGEM:IMPERIAL/2007/Notebook/2007-9-2

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Construction of pT7-GFP

  1. 5 colonies grew in the ligation plate
  2. All 5 colonies were picked and each was grown in 5 ml of LB + Kan
  3. The 5 cultures were incubated at 37°C overnight

Cloning of Biobricks (for Maxiprep)

  1. Placed 200 ml of LB in a 500 ml conical flask
  2. Innoculated 2 ml of culture into LB media in flask
  3. Incubated overnight at 37 °C

  • 2. BBa_I13422 [ptet-GFP]
  • 18. BBa_T9002 [plux-GFP]

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