IGEM:IMPERIAL/2009/Encapsulation/Recombinases

From OpenWetWare

Jump to: navigation, search

Contents

Killing strategy - Recombinases

Description

Recombinases are DNA-binding enzymes that recognise sites on the DNA and recombine them. By sprinkling the genome with Dif sequences and expressing the recombinase (see below for various ones) under an inducible promoter, we can select for the large scale excision of the genome. This would result in the killing of the bacterium.

The Dif sequence excision mechanism
The Dif sequence excision mechanism

E. Coli

B. Subtilis

  • CodV and RipX[1]


Will excise Dif sites resulting in the deletion of whatever is in between two sites.



Error fetching PMID 10498718:
  1. Error fetching PMID 10498718: [CodV_RipX]
Personal tools