Killing strategy - Recombinases
Recombinases are DNA-binding enzymes that recognise sites on the DNA and recombine them. By sprinkling the genome with Dif sequences and expressing the recombinase (see below for various ones) under an inducible promoter, we can select for the large scale excision of the genome. This would result in the killing of the bacterium.
- CodV and RipX
Will excise Dif sites resulting in the deletion of whatever is in between two sites.
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