M2 is the encapsulation phase. Upon detection of lowered glucose concentrations, the cells that have already produced the protein of interest will now be triggered to produce Colanic acid and Trehalose.
We have looked at 3 parts of the M2 phase:
- Using characteristics of the CRP promoter,
- Modelling how glucose consumption is linked to cell growth and using the drop in glucose concentration for indirect timing of encapsulation (via the CRP promoter)
- Modelling how the CRP promoter activity varies with different secondary carbon sources
- Protein production,
- Modelling Colanic acid and Trehalose production
M0 literature info
Old stuff to be tidied
2) Colanic Acid
Ultimatly, we want a model that will show how a PoPs input is related to the % of cells that remain intact following exposure to simulated gastric conditions. This can then be coupled to any PoPs characterised promoter to identify the minimal time required to complete encapsulation.
Characterisation of transfer functions is very important for this module. We hope to characterise the transfer functions of POPS in -> Trehalose out; and Trehalose Conc -> Protein Resistance (Catalase activity?).