IGEM:MIT/2005/Friday 9/7

Updates

This is a new article that seems like a relevant and interesting review of antibody engineering, describing scFvs in particular File:EngineeredAbs NatBiot05.pdf

General=

• There's a microscope in TK's lab in addition to Drew's
• Access to bld.68, lab and office off hours - will be done Wed. when Drew gets back
• Lab space: Got it all worked out, I'll show everyone around after the meeting.

Subteam Updates

Input-Maxine

None

Head Unit-Jen

• BurningMan was awesome
• Will continue to work on scFv-test construct & testing
  • Personally, would like to help with more urgent aspects of project

ToxR-Will

• Assemblies

Been using 3-drug method

• ToxR is toxic in cells?

FecA-Annie

• Construction of FecA Pro-RFP, FecA Pro-GFP

Verifying step

• FecA'

Tried 3-step PCR method but last step failed

Gel diagnosis suggests connection step fails --> advisors?

PLAN: redo 3rd step

• Fur & FecA KO

PCR CmR and TetR using new primers, only has CmR about right size, TetR controls indicated primer dimers

PLAN: Proceed with Fur KO

Redesign primers for PCR TetR

• Sequences have come in

scFv-Linker is correct

Planning

• Scheduling

-With Advisors

meeting - change date and time

Lab moving

-With Team

meeting - date and time

Team coordination