IGEM:MIT/2007/Notebook/2007-7-6

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Agenda

  1. Grow overnight liquid cultures of parts B0034. F2620, and E1010
  2. Request merR and Pmer from UGA

Last night's plates

First check at 9AM (~14 hrs incubation)

  • Very small colonies because of: stacked in incubation room? bad transformation? fewer uL's on some plates? haven't incubated long enough?
Part         Plate        # Colonies
B0034        1            12
             2            20
             3            7
F2620        1            2
             2            1
             3            7
E1010        1            2
             2            3 
             3            2

Transformed and plated another set

  • also did a positive control to make sure our cells can actually grow
  • Controls: 1 plate of untransformed on LB+Amp, 1 plate untransformed on LB
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