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Red light test

  • Double transformation that Alisa did was used as folows:
  • 2 plates (Amp/Kan/Chlor) had Xgal added (20 mg/mL) by disolving 1.6 mg of Xgal in 80 μL of DMSO
  • 1 colony streaked onto each plate
  • One plate wrapped in alluminium foil and placed in 37°C incubator
  • Other plate left on lab bench uncovered

Alex and Simon

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