IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/22

Cyanobacteria Mediated Filtration of Coal Flue Gas

Date: 08/22/13 People in lab: Emily Puleo, Hannah Frye

Title: Gel electrophoresis of PCR products from 8/21/13

Start Time: 3:00 PM

Purpose: To confirm presence of amplified genes, check for successful PCR

Protocol: LTM ed. 2

Well	1	2	3	4	5	6	7	8
Sample	Ladder	8/21/13 norCB A	8/21/13 nosZ A	8/21/13 hmp A

Results: Product visible for hmp, no product visible for nosZ or norCB

Notes: PCR hmp for TOPO-TA cloning, change temp of nosZ and norCB

Stop Time: 3:40 PM

Next: See notes